Spin labeling
Spin labeling the next millennium
edited by Lawrence J. Berliner
Publisher: New York : Kluwer Academic, c2002.
ISBN: 6610206430
DDC: 574.192028
LCC: QH324.9
Edition: (electronic bk.)
Notes:
Title from e-book title screen (viewed October 9, 2007).
Includes bibliographical references and index.
Cover -- Table of Contents -- Introduction: Reflections on the
Beginning of the Spin Labeling Technique -- Chapter 1 Analysis of
Spin Label Line Shapes with Novel Inhomogeneous Broadening from
Different Component Widths: Application to Spatially Disconnected
Domains in Membranes -- 1. Introduction -- 1.1. Inhomogeneous
Broadening Due to Unresolved Hyperfine Structure -- 1. 2.
Inhomogeneous Broadening Due to Different Component Widths . -- 2.
Theory -- 2.1. Sum of Two Spectra with Different Widths -- 2.2.
Statistical Distribution of Spin Labels -- 2.3. Methods to Determine
the Existence of a Statistical Distribution of Spin Labels -- 2.4.
Experimental Considerations -- 3. Applications -- 3.1. Domain
Structure in a Two-Component, Two-Phase Lipid Bilayer System -- 3.2.
Effect of a Peptide on Domain Structure -- 4. Conclusions --
References -- Chapter 2 Progressive Saturation and Saturation
Transfer EPR for Measuring Exchange Processes and Proximity Relations
in Membranes -- 1. Introduction -- 1.1. Continuous Wave Saturation --
1. 2. Effective Spin Lattice Relaxation Times -- 2. Contributions to
Intrinsic Spin-Lattice Relaxation Rates -- 2.1. Electron Relaxation
Mechanisms -- 2.2. Nuclear Relaxation Mechanisms -- 3. Paramagnetic
Relaxation Enhancement -- 3.1. Paramagnetic Enhancement by Heisenberg
Exchange -- 3.2. Paramagnetic Enhancement by Magnetic Dipole-Dipole
Interactions -- 4. Spin Lattice Relaxation Enhancement by Exchange
Processes -- 4.1. Two-Site Exchange -- 4.2. Heisenberg Spin Exchange
-- 5. Continuous Wave Saturation Measurements -- 5.1. Progressive
Saturation EPR -- 5.2. Saturation Transfer EPR -- 6. Applications --
6.1. Two-Site Lipid Exchange and Lipid-Protein Interactions -- 6.2.
Lipid and Protein Collision Frequencies: Translational Diffusion --
6.3. Spin Label Location and Accessibility: Paramagnetic Relaxation
-- 6.4. Distance Measurements: Dipolar Relaxation -- 6.5. Slow
Rotational Diffusion and Saturation Studies -- 7. Conclusions --
References -- Chapter 3 Comparative Spin Label Spectra at X-Band and
W-band -- 1. Introduction -- 2. Comparative X - and W-Band EPR
Spectra of Spin Labels in Isotropic Liquids -- 2.1. Solution of
Perdeuterated Tempone in Toluene -- 2.2. Solution of 3-Doxyl-17
Hydroxy-5 Androstane in O-xylene -- 2.3. Resolution Enhancement in
High Magnetic Fields: Mixtures of Phenyl-tert-butylnitrone Spin
Adducts -- 3. Sample Deoxygenation in CW HF EPR Experiments -- 4.
Comparative X - and W-Band EPR Spectra of Spin-Labeled Phospholipids
and Proteins -- 4.1. Small Spin Labels in Phospholipid Membranes --
4.2. Doxyl Stearic Acid Labels in Phospholipid Membranes -- 4.3.
Binding Spin-Labeled Fatty Acids to Bovine Serum Albumin -- 4.4.
Spin-Labeled Proteins: Immobilized Labels -- 4.5. Spin-Labeled
Proteins: Mobile Labels -- References -- Chapter 4 Use of Imidazoline
Nitroxides in Studies of Chemical Reactions: ESR Measurements of the
Concentration and Reactivity of Protons, Thiols, and Nitric Oxide --
1. Introduction -- 2. Stable Nitroxides in Proton Exchange Reactions
-- 2.1. Theoretical Considerations -- 2.2. Effect of pH on ESR
Spectra of Stable Nitroxides -- 2.3.pH-Sensitive Nitroxides in
Studies of Proton Exchange Reactions -- 2.4. ESR Measurements of the
Local Concentration of Protons -- 2.5. ESR Studies of Proton.
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