000 04162cam 2200301 a 4500
001 38438680
003 OCoLC
005 20020625183633.0
008 980203s1998 nju 001 0 eng
010 $a98012453
020 $a0896034941
020 $a0896033937
040 $aDLC$cDLC$dAGL$dNLM$dZR1
049 $aZR1A
090 $aQP 623 .R535$bR37 1998
245 00 $aRNA isolation and characterization protocols
/$cedited by Ralph Rapley and David L. Manning.
260 $aTotowa, N.J. :$bHumana Press,$cc1998.
300 $axiii, 264 p. ;$c24 cm.
440 0 $aMethods in molecular biology (Clifton, N.J.)
;$vv. 86.
504 $aIncludes bibliographical references and index.
505 00 $aTABLE OF CONTENTS -- $tIntroduction to isolating
RNA / Donald E. Macfarlane and Christopher E. Dahle --
$tLarge and small scale RNA preparations from eukaryotic
cells / Wolfgang Uckert, Wolfgang Walther and Ulrike Stein
-- $tAn improved rapid method of isolating RNA from cultured
cells / David B. Batt, Gordon G. Carmichael and Zhong Liu --
$tIsolating RNA with the cationic surfactant, Catrimox-14 /
Christopher Dahle and Donald E. Macfarlane -- $tRNA
extraction from formalin-fixed and paraffin-embedded tissues
/ Giorgi Stanta, Serena Bonin and Rosella Perin --
$tExtraction and purification of RNA from plant tissues
enriched in polycassharides / Shu-Hua Cheng and Jeffrey R.
Seeman -- $tIsolation of plant mitochondrial RNA from green
leaves / Fei Ye, Wolfgang O. Abel and Ralf Reski.
505 00 $aTABLE OF CONTENTS -- $tExtraction of RNA from
fresh and frozen blood / Bimal D. M. Theophilus --
$tIsolation of total RNA from bacteria / John Heptinstall --
$tIsolation of total RNA from tissues or cell lines :
visualization in gel / Tapas Mukhopadhyay and Jack A. Roth
-- $tIsolation of messenger RNA / Sian Bryant and David L.
Manning -- $tUV spectrometric analysis of ribonucleic acids
/ Ralph Rapley and John Heptinstall -- $tFormaldehyde gel
electrophoresis of total RNA / Sian Bryant and David L.
Manning -- $tPreparation of RNA dot-blots / Rachel Hodge --
$tNonradioactive northern blotting / Rainer Low -- The$tuse
of RNA probes for the analysis of gene expression : northern
blot hybridization and ribonuclease protection assay /
Dominique Belin -- $tAnalysis of RNA by northern blotting
using riboprobes / Rai Ajit K. Srivastava -- $tRNA
quantitative analysis from fixed and paraffin-embedded
tissues / Giorgi Stanta, Serena Bonin and Rene Utrera --
$tQuantitative analysis of RNA species by PCR and
solid-phase minisequencing / Anu Suomalainen and
Ann-Christine Syvanen.
505 00 $aTABLE OF CONTENTS -- $tPreparation of tissue
sections and slides for mRNA hybridization / Giorgi Terenghi
-- $tDetecting mRNA in tissue sections in
digoxigenin-labeled probes / Giorgi Terenghi -- $tOne-tube
RT-PCR with sequence specific primes / Ulrich Pfeffer --
$tIdentification of differentially exposed genes by
nonradioactive differential display messenger RNA / Thomas
C. G. Bosch and Jan U. Lohmann -- $tCharacterization of RNA
using continous RT-PCR coupled with ELOSA / Francois Mallet,
Guy Oriol and Bernard Mandrand -- $tGene expression analysis
by CD-RT-PCR / Eric de Kant -- $tPrimer extension analysis
of mRNA / Maggie Walmsley, Mark leonard and Roger Patient --
S1 mapping using single-stranded DNA probes / Stephanie
Viville and Roberto Mantovani -- $tMeasurments of rate of
transcription in isolated nuclie by nuclear run-off assay /
Rai Ajit K. Srivastava and Gustov Schonfeld --
$tTranscription in vitro using bacteriophage RNA polymerases
/ Elaine T. Schenhborn -- $tIn vitro translation of
messenger RNA in a wheat germ extract cell-fee system /
Louise Oliver, Anne Grobler-Rabie and Charles D. Boyd -- The
xenopus egg extract translation system / Glenn M. Matthews
and Alan Colman -- $tPurification and characterization of
viral dsRNA genome profiles by crosshybridization /
Lesley-Ann Martin and Peter P. C. Mertens.
650 0 $aRNA$xPurification$xLaboratory manuals.
650 0 $aRNA$xAnalysis$xLaboratory manuals.
650 12 $aRNA$xisolation & purification$vlaboratory
manuals.
700 1 $aRapley, Ralph.
700 1 $aManning, David L.
