Pichia Protocols
Pichia Protocols (Methods in Molecular Biology) James M. Cregg (Editor)
Publisher: Humana Press
ISBN: 1588294293
DDC: 660.63
Edition: Hardcover; 2007-08-08
Summary:
Pichia Protocols focuses on recent developments of Pichia pastoris as
a recombinant protein production system. Highlighted topics include a
discussion on the use of fermentors to grow Pichia pastoris,
information on the O - and N-linked glycosylation, methods for
labeling Pichia pastoris expressed proteins for structural studies,
and the introduction of mutations in Pichia pastoris genes by the
methods of restriction enzyme-mediated integration (REMI). This
volume fully updates and expands upon the first edition of Pichia
Protocols, and focuses primarily on information that has come to
light since its original publication. Each chapter presents
cutting-edge and cornerstone protocols for utilizing P. pastoris as a
model recomibinant protein production system.
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