Library: Commonwealth Scientific and Industrial Research Organization (CSIRO)
Last Loaded: 11/21/2003
MARC Timestamp: 05/21/2003
Control Number Org.:
Control Number: 248091
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020 $a0879695773 (pbk.)
020 $a0879695765 (hbk.)
035 $a(CaOOAMICUS)000022198357
035 $a248091
040 $aKIN$dQS:TCP$dVS:NAH
050 00 $aQH442.2$b.S26 2001
050 4 $aQH442.2$b.M26 2001
082 0 $a572.8$221
082 1 $a574.87/3224$219
100 1 $aSambrook, Joseph.
245 10 $aMolecular cloning :$ba laboratory manual
/$cJoseph Sambrook, David W. Russell.
250 $a3rd. ed.
260 $aCold Spring Harbor, N.Y. :$bCold Spring Harbor
Laboratory,$cc2001.
300 $a3 v. :$bill. ;$c28 cm.
504 $aIncludes bibliographical references and index.
505 0 $aCh.1 Plasmids and their usefulness in molecular
cloning -- Ch.2 Bacteriophage and its vectors -- Ch.3
Working with bacteriophage M13 vectors -- Ch.4 Working with
high capacity vectors -- Ch.5 Gel Electrophoresis of DNA and
pulsed-filed agarose -- Ch.6 Preparation and analysis of
eukaryotic genomic DNA -- Ch.7 Extraction, purification, and
analysis of mRNA from Eukaryotic cells -- Ch.8 In vitro
amplification of DNA by the polymerase chain reaction --
Ch.9 Preparation of radiolabeled DNA and RNA probes -- Ch.10
Working with synthetic oligonucleotide probes -- Ch.11
Preparation of cDNA libraries and gene identification --
Ch.12 DNA sequencing -- Ch.13 Mutagenesis -- Ch.14 Screening
expression libraries -- Ch.15 Expression on cloned genes in
escherichia coli -- Ch.16 Introducing cloned genes into
cultures mammalian cells -- Ch.17 Analysis of gene
expression in cultured mammalian cells -- Ch.18 Protein
interaction technologies.
506 $aOnline access available to those with print copy
520 $a"A major goal of all three editions of Molecular
Cloning has been to provide researchers with up-to-date
protocols that work reproducibly. Users of the previous
editions will recognize many of the organizational features
in the experimental sections of this book. Nevertheless,
the revision of the text has been extensive and detailed.
Ancient protocols have been modernized, while new protocols
have been added to reflect the continuing penetration of
molecular cloning into almost all areas of biomedical
research. Of equal importance has been our desire to
explain how and why particular methods work, and with
reasoned arguments for choosing between alternative
procedures."--Preface (p. xxi).
650 0 $aMolecular cloning$xLaboratory manuals.
650 0 $aEukaryotic cells$xLaboratory manuals.
650 10 $aCloning, Molecular$vLaboratory Manuals.
700 1 $aRussell, David W.
710 2 $aCold Spring Harbor Laboratory.
