"A major goal of all three editions of Molecular Cloning has been to
provide researchers with up-to-date protocols that work reproducibly.
Users of the previous editions will recognize many of the
organizational features in the experimental sections of this book.
Nevertheless, the revision of the text has been extensive and
detailed. Ancient protocols have been modernized, while new protocols
have been added to reflect the continuing penetration of molecular
cloning into almost all areas of biomedical research. Of equal
importance has been our desire to explain how and why particular
methods work, and with reasoned arguments for choosing between
alternative procedures."--Preface (p. xxi).
Notes:
Includes bibliographical references and index.
Online access available to those with print copy
Ch.1 Plasmids and their usefulness in molecular cloning -- Ch.2
Bacteriophage and its vectors -- Ch.3 Working with bacteriophage M13
vectors -- Ch.4 Working with high capacity vectors -- Ch.5 Gel
Electrophoresis of DNA and pulsed-filed agarose -- Ch.6 Preparation
and analysis of eukaryotic genomic DNA -- Ch.7 Extraction,
purification, and analysis of mRNA from Eukaryotic cells -- Ch.8 In
vitro amplification of DNA by the polymerase chain reaction -- Ch.9
Preparation of radiolabeled DNA and RNA probes -- Ch.10 Working with
synthetic oligonucleotide probes -- Ch.11 Preparation of cDNA
libraries and gene identification -- Ch.12 DNA sequencing -- Ch.13
Mutagenesis -- Ch.14 Screening expression libraries -- Ch.15
Expression on cloned genes in escherichia coli -- Ch.16 Introducing
cloned genes into cultures mammalian cells -- Ch.17 Analysis of gene
expression in cultured mammalian cells -- Ch.18 Protein interaction
technologies.
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