Microbial gene essentiality
Microbial gene essentiality: protocols and bioinformatics
edited by Andrei L. Osterman and Svetlana Y. Gerdes
Publisher: Totowa, N.J. : Humana Press, c2008.
ISBN: 1597453218
LCC: QH434
Edition: (e-ISBN)
Notes:
Includes bibliographical references and index.
Overview of whole-genome essentiality analysis / Karen Joy Shaw --
Pt. I. Experimental protocols. IA. Populational genome-wide
essentiality screens. Transposon-based strategies for the
identification of essential bacterial genes / William S. Reznikoff
and Kelly M. Winterberg -- Identification and analysis of essential
genes in Haemophilus influenzae / Sandy M.S. Wong and Brian J.
Akerley -- Transposon site hybridization in Mycobacterium
tuberculosis / Jeffrey P. Murry ... [et al.] -- Essential genes in
the infection model of Pseudomonas aeruginosa PCR-based
signature-tagged mutagenesis / François Sanschagrin, Irena
Kukavica-Ibrulj, and Roger C. Levesque -- Whole-genome detection of
conditionally essential and dispensable genes in Escherichia coli via
genetic footprinting / Michael D. Scholle and Svetlana Y. Gerdes --
Generating a collection of insertion mutations in the Staphylococcus
aureus genome using bursa aurealis / Taeok Bae ... [et al.] --
Multipurpose transposon insertion libraries for large-scale analysis
of gene function in yeast / Anuj Kumar --
IB. Systematic collections of knockout mutants. How to make a defined
near-saturation mutant library. Case 1: Pseudomonas aeruginosa PAO1 /
Michael A. Jacobs -- Comparing insertion libraries in two Pseudomonas
aeruginosa strains to assess gene essentiality / Nicole T. Liberati
... [et al.] -- The construction of systematic in-frame, single-gene
knockout mutant collection in Escherichia coli K-12 / Tomoya Baba and
Hirotada Mori -- The applications of systematic in-frame, single-gene
knockout mutant collection of Escherichia coli K-12 / Tomoya Baba ...
[et al.] -- A novel, simple, high-throughput method for isolation of
genome-wide transposon insertion mutants of Escherichia coli K-12 /
Takeyoshi Miki, Yoshihiro Yamamoto, and Hideo Matsuda --
High-throughput creation of a whole-genome collection of yeast
knockout strains / Angela M. Chu and Ronald W. Davis -- Analysis of
genetic interactions on a genome-wide scale in budding yeast:
diploid-based synthetic lethality analysis by microarray / Pamela B.
Meluh ... [et al.] --
IC. Genome minimization. Scarless engineering of the Escherichia coli
genome / Tamás Fehér ... [et al.] -- Minimization of the Escherichia
coli genome using the Tn5-targeted Cre/loxP excision system / Byung
Jo Yu and Sun Chang Kim -- Construction of long chromosomal deletion
mutants of Escherichia coli and minimization of the genome / Jun-ichi
Kato and Masayuki Hashimoto -- ID. Conditional knockouts.
Identification of essential genes in Staphylococcus aureus by
construction and screening of conditional mutant library / Dezhong
Yin and Yinduo Ji -- Techniques for the isolation and use of
conditionally expressed antisense RNA to achieve essential gene
knockdowns in Staphylococcus aureus / Allyn Forsyth and Liangsu Wang
-- Introduction of conditional lethal amber mutations in Escherichia
coli / Christopher D. Herring -- Pt. II. Bioinformatics. IIA.
Statistics. Statistical methods for building random transposon
mutagenesis libraries / Oliver Will -- Statistical evaluation of
genetic footprinting data / Gábor Balázsi -- Modeling competitive
outgrowth of mutant populations: why do essentiality screens yield
divergent results? / Alexander I. Grenov and Svetlana Y. Gerdes --
Statistical analysis of fitness data determined by TAG hybridization
on microarrays / Brian D. Peyser, Rafael Irizarry, and Forrest A.
Spencer -- IIB. Data integration and modeling. Profiling of
Escherichia coli chromosome database / Yukiko Yamazaki, Hironori
Niki, and Jun-ichi Kato -- Gene essentiality analysis based on DEG, a
database of essential genes / Chun-Ting Zhang and Ren Zhang --
Detection of essential genes in Streptococcus pneumoniae using
bioinformatics and allelic replacement mutagenesis / Jae-Hoon Song
and Kwan Soo Ko -- Design and application of genome-scale
reconstructed metabolic models / Isabel Rocha, Jochen Förster, and
Jens Nielsen -- Predicting gene essentiality using genome-scale in
silico models / Andrew R. Joyce and Bernhard Ø. Palsson --
Comparative approach to analysis of gene essentiality / Andrei L.
Osterman and Svetlana Y. Gerdes.
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