This is the first manual presenting a set of protocols for production
of doubled haploids (DH) in 22 major crop plant species including 4
tree species. It contains various protocols and approaches of DH
production proven for different germplasm of the same species. The
protocols describe in detail all steps of DH production - from donor
plants growth conditions, through in vitro procedures, media
composition and preparation, to regeneration of haploid plants and
chromosome doubling methods. The users of this manual will be able to
choose among microspore versus anther cultures, wide hybridisation or
gynogenesis, the most suitable method for production of DH in
particular laboratory conditions for their plant material. Numerous
colour illustrations should help in this decision. The user will also
find information on organization of a doubled haploid laboratory,
basic DH media and on associated simple cytogenetic methods for
ploidy level analysis. The practical protocols are supplemented with
the list of published DH protocols for other crop plants and separate
chapters dealing with major applications of DH in breeding, mutant
production, transgenesis, and genetic mapping and genomics. The book
was prepared by the Plant Breeding and Genetics Section of the Joint
FAO/IAEA Division of Nuclear Techniques in Food and Agriculture in
close co-operation with the EU program `COST 851' on `Gametic cells
and molecular breeding for crop improvement'.
Color Atlas of Turfgrass Weeds
(L. B. McCarty, J. W. Everest, D. W. Hall, T. R. Murphy, F. Yelverton; ISBN: 1575041421;
Hardcover; 2001-01-01; 71% match)
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