DNA sequencing III
DNA sequencing III: dealing with difficult templates
edited by Jan Kieleczawa
Publisher: Sudbury, Mass. : Jones and Bartlett Publishers, c2009.
ISBN: 076374297X
DDC: 572.86
LCC: QP624
Edition: (alk. paper)
Notes:
Includes bibliographical references and index.
Controlled heat-denaturation of DNA plasmids and PCR fragments --
Heat denaturation is an effective step in sequencing of many
difficult DNA templates -- Solutions for sequencing difficult regions
-- Improving sequence results from difficult templates with Phi 29
DNA polymerase and nucleotide analogs: the TempliPhi sequence
resolver kit -- Sequencing through various secondary structures:
detailed studies of pDEST vectors and other DNA templates with
hairpins -- Transcriptional sequencing as a tool for reading
difficult-to-read templates -- Bias-free cloning of "unclonable" DNA
for simplified genomic finishing -- Bioinformatics tools to aide
sequencing of difficult templates.
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