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010 $a 2005022077
020 $a0879697725 (hardcover : alk. paper)
020 $a0879697717 (pbk. : alk. paper)
020 $a9780879697716
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050 00 $aQH442.2$b.S24 2006
082 00 $a572.8$222
100 1 $aSambrook, Joseph.
245 14 $aThe condensed protocols from Molecular cloning :
a laboratory manual /$cJoseph Sambrook, David W. Russell.
260 $aCold Spring Harbor, N.Y. :$bCold Spring Harbor
Laboratory Press,$cc2006.
300 $av, 800 p. :$bill. ;$c28 cm.
500 $a"A single-volume adaptation of the three-volume
third edition of Molecular cloning: a laboratory manual"--P.
[4] of cover.
504 $aIncludes bibliographical references and index.
505 0 $aPlasmids and their usefulness in molecular
cloning -- Bacteriophage and its vectors -- Working with
bacteriophage M13 vectors -- Working with high-capacity
vectors -- Gel electrophoresis of DNA and pulsed-field
agarose gel electrophoresis -- Preparation and analysis of
eukaryotic genomic DNA -- Extraction, purification, and
analysis of mRNA from eukaryotic cells -- In vitro
amplification of DNA by the polymerase chain reaction --
Preparation of radiolabeled DNA and RNA probes -- Working
with synthetic oligonucleotide probes -- Preparation of cDNA
libraries and gene identification -- DNA sequencing --
Mutagenesis -- Screening expression libraries -- Expression
of cloned genes in escherichia coli -- Introducing cloned
genes into cultured mammalian cells -- Analysis of gene
expression in cultured mammalian cells -- Protein
interaction technologies.
650 0 $aMolecular cloning$vLaboratory manuals.
650 2 $aCloning, Molecular$vLaboratory Manuals.
700 1 $aRussell, David W.$q(David William),$d1957-
700 1 $aSambrook, Joseph.$tMolecular cloning.
856 41 $3Table of contents$uhttp://www.loc.gov/catdir/toc/
ecip0517/2005022077.html
