"A single-volume adaptation of the three-volume third edition of
Molecular cloning: a laboratory manual"--P. [4] of cover.
Includes bibliographical references and index.
Plasmids and their usefulness in molecular cloning -- Bacteriophage
and its vectors -- Working with bacteriophage M13 vectors -- Working
with high-capacity vectors -- Gel electrophoresis of DNA and
pulsed-field agarose gel electrophoresis -- Preparation and analysis
of eukaryotic genomic DNA -- Extraction, purification, and analysis
of mRNA from eukaryotic cells -- In vitro amplification of DNA by the
polymerase chain reaction -- Preparation of radiolabeled DNA and RNA
probes -- Working with synthetic oligonucleotide probes --
Preparation of cDNA libraries and gene identification -- DNA
sequencing -- Mutagenesis -- Screening expression libraries --
Expression of cloned genes in escherichia coli -- Introducing cloned
genes into cultured mammalian cells -- Analysis of gene expression in
cultured mammalian cells -- Protein interaction technologies.
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