Combinatorial Genetic Logic: Multiple transcription factor regulation in synthetic bacterial circuits
This book examines several synthetic transcriptional regulatory circuits in bacteria. Chapter 1 presents a novel technique for shuffling short sequences of regulatory DNA to generate exponential libraries of functional sequences. Chapter 2 introduces a library of combinatorial promoters--genetic logic gates which integrate multiple environmental signals. Chapter 3 examines the role of genetic noise in transcriptional co-regulation using a three-color fluorescent reporter system. Chapter 4 showcases a synthetic circuit for the control of population density in a bacterial growth culture. These examples highlight a quantitative approach for characterizing the logical input functions of prokaryotic genes, focusing on the relationship between combinatorial promoter functions and architectures.
- Physical Description: 6.3"x8.9"x0.6"; 0.7 lb; 220 pages
- Edition Info: Paperback; 2009-11-16
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