General methods -- Bacterial strains and cloning vectors -- Enzymes
that modify DNA and RNA -- In vitro amplification of DNA using the
polymerase chain reaction (PCR) and the thermostable Taq DNA
polymerase -- DNA restriction fragment analysis and preparation -- In
vitro labeling of probes and filter hybridization -- Plasmid DNA
preparation from E coli hosts -- Preparation of DNA from lambda
bacteriophage clones -- Subcloning fragments into plasmid vectors and
plasmid construction -- Preparation of genomic DNA -- Preparation and
analysis of RNA from eukaryotic cells -- Genomic cloning --
Preparation and analysis of cDNA libraries -- Preparation of
subtractive cDNA -- Chain termination sequencing -- Transfection of
mammalian cells -- Basic methods of protein analysis -- In situ
hybridization -- Transgenic mouse preparation -- Detection and in
vitro generation of specific mutations in genes and cDNAs --
Preparation of stock solutions -- Generally useful information --
Suppliers of reagents and equipment.
Book Details:
Language: eng
Physical Description: xiv, 777 p. : ill. ; 26 cm.
Edition Info: $43.96
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