This ground-breaking atlas of oocyte ultrastructure forms a practical
guide to assisted reproduction. Extensively illustrated with
transmission electron microscopic (TEM) images, it contains chapters
on oocyte maturation, fertilization, fertilization failure, pitfalls
and technical problems of intracytoplasmic sperm injection, and
failed fertilization in vitro to explain the principles and
evaluation of TEM images. A large chapter of TEM images of the
ultrastructure of human oocytes illustrates fertilization failures
after ICSI and IVF and the ultrastructure of germinal vesicle oocytes
and metaphase I oocytes. It also contains detailed information about
abnormal metaphase II oocytes with sections on autolysis,
multi-vacuolated mature oocyte, refractile body, and fragmented
oocyte. The concluding chapter describes materials and methods for
single-cell transmission electron microscopy of the human oocyte.
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