Microarray technology is more accessible than ever, and an
ever-widening field of scientists is using this technology. However,
the manufacture, experimental design, and analysis of microarrays are
not always straightforward, and researchers new to the field run into
technical and theoretical roadblocks that can hinder progress with
this powerful new technology. A Beginner's Guide to Microarrays
addresses two audiences - the core facility manager who produces,
hybridizes, and scans arrays, and the basic research scientist who
will be performing the analysis and interpreting the results. User
friendly coverage and detailed protocols are provided for the
technical steps and procedures involved in many facets of microarray
technology, including: Cleaning and coating glass slides, Designing
oligonucleotide probes, Constructing arrays for the detection and
quantification of different bacterial species, Preparing spotting
solutions, Troubleshooting spotting problems, Setting up and running
a core facility, Normalizing background signal and controlling for
systematic variance, Designing experiments for maximum effect,
Analyzing data with statistical procedures, Clustering data with
machine-learning protocols. This book is addressed to researchers
using microarrays for the first time. One faces a myriad of problems
at the outset of such a task, and there is no need to 'reinvent the
wheel' for each scientist that runs into these problems. Knowing the
strengths and weaknesses of microarrays before research begins can
save time, money, and resources.
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