2D PAGE: Sample Preparation and Fractionation: Volume 2
2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology) Anton Posch (Editor)
Publisher: Humana Press
ISBN: 1603272097
DDC: 572
Edition: Hardcover; 2008-03-19
Summary:
The complex nature of proteins often requires a multitude of sample
preparation options. In 2D PAGE: Sample Preparation and
Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad
coverage of the principles and recent developments of sample
preparation and fractionation tools in Expression Proteomics and
specifically for two-dimensional electrophoresis (2-DE). Detailed
laboratory methods describe the process of sample fractionation used
to reduce the complexity of the protein sample and the methods of
2-DE analysis and a broad variety of gel-free mass spectrometry-based
technologies to detect previously undetectable proteins. Vital
information regarding protein isolation and preparation for
reproducible proteome analysis previously overlooked by scientists
has been clearly laid out in this pioneering two-volume set. Volume 2
illuminates specific applications of sample preparation tools and
fractionation strategies to study a variety of biological systems.
Invaluable for students of Biochemistry, Biomedicine, Biology and
Genomics and essential for the practicing scientists, 2D PAGE: Sample
Preparation and Fractionation, Volumes 1 & 2 delves into the study of
proteins with comprehensive and in depth methods and protocols to
provide a greater understanding of this intricate and complicated
field.
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