2D PAGE: Sample Preparation and Fractionation: Volume 1
2D PAGE: Sample Preparation and Fractionation: Volume 1 (Methods in Molecular Biology) Anton Posch,
Publisher: Humana Press
ISBN: 1588297225
DDC: 572
Edition: Hardcover; 2008-03-19
Summary:
The complex nature of proteins often requires a multitude of sample
preparation options. In 2D PAGE: Sample Preparation and
Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad
coverage of the principles and recent developments of sample
preparation and fractionation tools in Expression Proteomics and
specifically for two-dimensional electrophoresis (2-DE). Detailed
laboratory methods describe the process of sample fractionation used
to reduce the complexity of the protein sample and the methods of
2-DE analysis and a broad variety of gel-free mass spectrometry-based
technologies to detect previously undetectable proteins. Vital
information regarding protein isolation and preparation for
reproducible proteome analysis previously overlooked by scientists
has been clearly laid out in this pioneering two-volume set. Volume 1
focuses on the basics of sample preparation and protein labeling
techniques with an emphasis on fractionation by chemical reagents,
chromatography, and electrophoresis methods. The inclusion of
meticulous enrichment strategies further aids the protein researcher.
Invaluable for students of Biochemistry, Biomedicine, Biology and
Genomics and essential for the practicing scientists, 2D PAGE: Sample
Preparation and Fractionation, Volumes 1 & 2 delve into the study of
proteins with comprehensive and in depth methods and protocols to
provide a greater understanding of this intricate and complicated
field.
Lipid and Protein Traffic: Pathways and Molecular Mechanisms
(France) NATO Study Institute "Molecular Mechanisms of Lipid and Protein Traffic" (1997 : Cargese, North Atlantic Treaty Organization. Scientific Affairs Division (Corporate Author), Jos A. F. Op Den Kamp (Editor); ISBN: 3540641157;
Hardcover; 1998-06; 69% match)
•
Molecular Microbiology
(NATO Advanced Study Institute "Molecular Microbiology" (1997 : University of Birmingham), Stephen J. W. Busby (Editor), Christopher M. Thomas (Editor), Nigel L. Brown (Editor); ISBN: 3540638733;
Hardcover; 1998-01; 58% match)
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